Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) provided a foundational method to study chromatin structure by mapping the location of histone post-translational modifications (PTMs) and chromatin-binding proteins genome-wide. However, the innate challenges of ChIP-seq -- including poor sensitivity, low throughput, and high costs -- have severely limited the application of chromatin profiling, particularly as it relates to the study of rare/precious cell populations, drug development, and biomarker discovery.

But, there is a solution. By utilizing a novel in situ strategy that enables unprecedented mapping sensitivity and simplified workflows, CUT&RUN and CUT&Tag are creating a paradigm shift in epigenomics research. This presentation will illustrate how these scalable technologies unlock the full potential of epigenomics for next-generation chromatin studies.