December 11, 2020 -- Twist Bioscience has launched its new clonal-ready gene fragments. The gene fragments, with and without adapters, can be used to rapidly build constructs and minimize the time and cost of screening for clones.
Twist's gene fragments begin as oligonucleotides synthesized on the company's semiconductor-based silicon platform. The oligonucleotides are then annealed with polymerase chain reaction (PCR)-amplified to produce double-stranded DNA fragments. The fragments are error-corrected through an enzymatic reaction process. The final products are ready-to-use gene fragments that are compatible with many applications including cloning, gene and protein expression, pathway and enzyme engineering, and enzyme optimization.
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