June 23, 2021 -- Qiagen has rolled out its QIAprep & CRISPR Kit and CRISPR Q-Primer Solutions. The products are intended to support researchers in analyzing edited genetic material to identify how their interventions have changed the function of the DNA sequence in question.
The products provide scientists an all-in-one process for characterizing knockouts generated from guide RNA (gRNA) and knockins from small insertions during gene editing. The kit and Q-Primer solutions combine liquid-based sample preparation with polymerase chain reaction (PCR) detection and the Sanger method of DNA sequencing. Qiagen's new products can be used to analyze cells edited with methods such as CRISPR with adherent and suspension cell cultures and can be used in experiments with the proteins Cas9 and Cas12a or single-cell inputs.
Positive PCR controls for humans, mice, and rats are included to determine the effectiveness of the gene editing conditions.
The kit and solutions will also decrease the time to result in experiments, with cell cultivation requirements reduced by seven days compared with existing methods, Qiagen said.