New England Biolabs launches NEBNext immune sequencing kits

By Amerigo Allegretto, The Science Advisory Board contributing writer

New England Biolabs has launched NEBNext immune sequencing kits, which are available for human or mouse samples to allow for B-cell and/or T-cell repertoire analysis of infections, the company announced.

The kits allow for profiling somatic mutations in the full-length immune gene repertoires of B cells and T cells through the expression of complete antibody chains. The company said the kits include modular primer sets, providing information for complete V, D, and J segments and full isotype information analysis (immunoglobulin M [IgM], IgD, IgG, IgA, and IgE), as well as T-cell receptor (TCR) chain characterization.

The kits include a bioinformatic workflow on the company's Galaxy platform, which is based on the open-source Presto toolkit.

A template-switching unique molecular identifier-based mRNA barcoding process allows polymerase chain reaction copies derived from an individual molecule to be converted to a consensus sequence. The company said this improves sequence accuracy and eliminates reaction bias.


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