Synthego introduces new CRISPR light control tech

By The Science Advisory Board staff writers

October 7, 2020 -- Synthego has designed a new foundational technology for standardized precision and control of CRISPR-based genome editing inside cells using light.

The methodology, described in Nature Communications, involves a new class of guide molecules, called CRISPRoff. These synthetic single guide RNA (sgRNA) fragment in response to light, enabling precise temporal and spatial control of double-strand breaks created during gene editing. The work was done in collaboration with the U.S. National Institute of Standards and Technology Genome Editing Consortium to develop standards for precise and reproducible gene editing in therapeutic applications.

The in vitro research demonstrated that CRISPRoff is effective across multiple genomic targets in multiple cell lines and has the potential to reduce off-target events, without impacting genome editing efficiency.

CRISPRoff does not require additional engineering steps or modifications to nucleases, therefore it can fit within existing CRISPR gene editing workflows. It can also be tuned to different wavelengths of light, allowing for multiplexed genome editing. It is produced in a good manufacturing practice (GMP)-grade format.

Genetics Research licenses tech to Oxford Nanopore
Genetics Research has licensed certain of its patents related to its proprietary CRISPR-associated technologies for targeted DNA enrichment to Oxford...
Making CRISPR safer with new enzyme-based prediction tool
A new technique will help scientists choose the best available gene editing option for any given indication, making CRISPR technology safer, cheaper,...
Customized gene therapies successfully target rare eye diseases
Customized gene therapies successfully target rare eye diseases
New gene therapy destroys latent oral herpes in mice
Researchers recently reported that they were able to eliminate latent herpes simplex virus 1 in mice using a new gene editing technique that targets the...
New CRISPR-repressor system improves efficiency of gene therapies
Researchers have developed a CRISPR-based system that can simultaneously provide transcriptional control and gene editing on demand to improve the efficacy...

Copyright © 2020

Building Biology in 3D Hybrid Symposium
October 26-27
La Jolla, California United States
Northeast Regional Laboratory Staff and Core Directors (NERLSCD) 2021
November 3-5
Portsmouth, New Hampshire United States
CPhI Worldwide 2021
November 9-11
Milan, Lombardia Italy
Society for Immunotherapy of Cancer (SITC) Annual Meeting
November 10-14
Washington, DC, District of Columbia United States
Science Advisory Board on LinkedIn
Science Advisory Board on Facebook
Science Advisory Board on Twitter