The new assay includes reagents and tools to simplify workflows from sample preparation to data analysis and offers researchers an efficient way to obtain relevant data and shorten the time from biological question to answer, Cytek said.

Cytek's reagent assay is furnished with protocols for sample preparation, staining, and acquisition, as well as experiment and analysis templates to streamline workflow and improve laboratory efficiency, the company said.

The assay can help distinguish different subsets of immune cells, including regulatory, naive, activated, memory, and effector T cells; naive and memory B cells; early, mature, and terminal NK cells; and nonclassical and classical monocytes. Reagent kits function as a backbone upon which users can add additional markers and expand the panel, Cytek said.

In addition, to support the new 25-color immunoprofiling assay, Cytek has released an 18-color cFluor reagent kit that can be used with seven reagents from other suppliers. The 25-color assay has been optimized and titrated for use with seven Brilliant Violet reagents available from BioLegend for analyzing human peripheral blood mononuclear cells and whole blood on Cytek's full-spectrum systems.