Horizon Discovery accelerates CRISPR gene editing workflows

By The Science Advisory Board staff writers

July 7, 2020 -- Horizon Discovery Group has introduced a new stably expressing Cas9 cell line for knockout experiments and a dCas9-VPR cell line for gene activation experiments.

The products are designed to work with Horizon's Edit-R predesigned synthetic single guide RNA and CRISPR guide RNA to create streamlined CRISPR gene editing and modulation workflows. The cell lines are generated using Edit-R lentiviral particles with a blasticidin resistance cassette.

Both lines are available in the same background to enable loss- and gain-of-function studies without the need to engineer specific cell lines. The time and money saved by removing this step will help researchers increase productivity and simplify CRISPR workflows.

The complete package of quality control-validated cell lines with Edit-R predesigned single guide RNA products and DharmaFect transfection reagents will be bundled for sale.

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