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Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

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Immunology
10/16/2003 IMMUNOCYTOCHEMISTRY
10/16/2003 Direct Immunofluorescence Labeling
10/16/2003 DNA Staining for Cell Cycle Analysis By flow Cytometry
10/17/2003 CD3/ CD4 /CD8 T cell subset counts
10/17/2003 Flow Cytometric Analysis Of Bcl Family members
10/18/2003 Immunodetection of cyclin D1 and D2/D3 using flow cytometry
10/18/2003 Bioassay IFN-gamma
10/21/2003 The Under-Agarose Migration Assay
10/27/2003 CPP32 Activity Assay Using CPP32 Fluorogenic Substrate
10/27/2003 Quantitative Fluorescent Assay for the Simultaneous Identification of Apoptotic and Necrotic Cells
10/27/2003 Telomeric Repeat Amplification Protocol (TRAP) Telomerase Assay
10/27/2003 Raf-1 Kinase Assay
10/27/2003 JNK/SAP Kinase Assay
10/29/2003 Antigen-specific intracellular Calcium flux measurement
10/29/2003 ELISA for IFN-gamma and IL-10
10/29/2003 ELISPOT for IFN-gamma and IL-10
11/24/2003 Activation of apoptotic activity in cytoplasmic extracts and measurement of the resulting caspase enzymatic acticity
11/24/2003 Activation of apoptotic activity in cytoplasmic extracts for western blot analysis
11/24/2003 Analysis of apoptotic activity by qualitative DNA laddering assay and DAPI staining.
11/24/2003 ApoptosisReconstitution of activated cytoplasmic extracts with isolated radioactive nuclei.
11/24/2003 Preparation of cytoplasmic extracts for the application in a cell-free system
11/24/2003 Isolation of cell nuclei for the application in the cell-free system
5/3/2004 Antibody Purification
5/3/2004 Purification of Endotoxin Free mAbs
5/3/2004 Antibody Purification Protocol
5/3/2004 Affinity Purification of Antibodies Using Proteins Bound to Nitrocellulose
5/3/2004 Antibody Conjugation
5/3/2004 Antibody Staining of C. elegans
5/3/2004 Protocol for Metal Enhanced DAB Staining using HRP 2° Antibodies
5/3/2004 Preabsorbing Antisera with C. elegans Acetone Powder
5/5/2004 FACS STAINING OF PBLs for monitoring CD4 and CD8 depletion
5/5/2004 Protocol for intracytoplasmic staining of cytokines for FACS analysis
4/29/2005 In vivo CTL assay
7/27/2005 ISOLATION AND CULTURE OF MOUSE BONE MARROW-DERIVED MACROPHAGES
11/21/2005 immunofluorescence
12/20/2005 Cr Release Cytotoxicity assay
12/22/2005 Assays of E. coli Aggregation by Hemocyte Immune Proteins. LPS-Triggered Hemocyte Spreading and Immune-Protein Release.
2/14/2008 Isolation and staining of mouse PP lymphocytes for FACS
2/15/2008 NK Cell Assay by Fluorimetric Method
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