Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

To comment on these protocols, please participate in the ongoing "Protocol Reviews" discussion in The Science Advisory Board's Forum.


General Lab
10/1/2003	Alkaline Phosphatase And Phospholipase C Assays
10/16/2003	TRANSFECTION OF MAMMALIAN CELLS USING LIPOFECTAMINE REAGENT
10/18/2003	Fluorescein labeling of proteins
10/18/2003	Dynamic Flow Assay in a Parallel Plate Flow Chamber
10/18/2003	Bioassay TNF-alpha
10/19/2003	Ultraviolet (UV) Photochemical Crosslinking to Detect RNA Binding Proteins
10/19/2003	Gel Shift with Glutaraldehyde
10/24/2003	Media for the Growth of Bactertial Symbionts for Entomopathogenic Nematodes
10/24/2003	Shortcut Yeast transformation
11/7/2003	ARABIDOPSIS TRANSFORMATION
11/7/2003	Pollen Development in Anthers of Arabidopsis
11/10/2003	BAC DNA Fingerprint Analysis
11/18/2003	Sporulation Protocol
11/18/2003	Sporulation Protocol 2
11/18/2003	Yeast Vivisection
11/18/2003	Quantitative Mating Assay
11/18/2003	Yeast IF with Methanol/Acetone Dehydration
11/18/2003	Yeast IF without Dehydration
11/18/2003	Yeast Cell Cycle by Flow Cytometry
11/18/2003	Rhodamine-Phalloidin/Calcofluor Staining
11/18/2003	Rhodamine-Phalloidin/Calcofluor Staining
11/19/2003	Silver Staining of SDS-PAGE gels
11/19/2003	Cosmid Cloning: Cell preparation, DNA packaging, and Cell Transfection
11/20/2003	Demonstration of endospores by means of the Shaeffer-Fulton endospore stain
11/21/2003	Determination of Chlorophyll Content in Chloroplasts
11/21/2003	Isolation of RNA from Tobacco Mosaic Virus (TMV)
11/21/2003	Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
11/21/2003	Terminal dUTP Nick End-Labeling (TUNEL) of Barley Aleurone Protoplasts
11/21/2003	BACTERIAL AGGLUTINATION:
11/21/2003	PASSIVE AGGLUTINATION
11/21/2003	Gram-staining Procedure
11/21/2003	SMEAR PREPARATION
11/21/2003	Fluorescent - Auramine Rhodamine Staining For Mycobacterium
11/21/2003	Ziehl-Neelsen Staining Procedure For Mycobacterium
11/24/2003	Llewellyn's Double Oxidation, Thiosemicarbazide, Schmorl
11/24/2003	Goldman's Iron Hematoxylin
11/24/2003	Oxidase (Spot Test Dropper)
11/24/2003	Urea Slant
1/29/2004	CGH Protocol-Metaphase chromosome preparation
1/29/2004	CGH Protocol
1/29/2004	CGH Protocol-DNA labeling by nick translation
5/3/2004	Preparing Serum from Whole Blood
5/5/2004	Immunofluroescence Technique
5/5/2004	Post-Embedding Immunogold Method
6/2/2004	Rapid disruption of bacterial colonies to test the size of plasmids.
6/2/2004	Making Mg++ /Ca++ competent E.Coli BL21 cells
6/7/2004	Preparation of competent cells.
3/1/2005	Quantitative Determination of Sulfhydryl (-SH) Groups in Peptides and Proteins
6/21/2005	glycosaminoglycans purification
3/29/2006	In house Competent Cell preparation
6/1/2006	Determination of the Extinction Coefficient for a Protein of Unknown Concentration
12/8/2006	Retina whole mounts from chicken embryos
1/16/2008	May Grun Wald Giemsa staining protocol for Bone marrow smears

Join Today

Scientific & Medical
Experts Needed!

The Science Advisory Board is the world's most established network of life scientists!

Voice your opinions on companies, products, protocols and even humor in a lively, real-time, interactive Online Community of over 49,000 life science & medical professionals.

Redeem generous rewards for participation in studies, contributing website content and referring colleagues.

Join Right Now!
(It's Free!)

Search This Site

Get Connected

The Science Advisory Board Linkedin Group