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Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

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DNA Transformation/Library Preparation
10/8/2003 DNA ligation  
10/8/2003 Competent cell preparation  
10/16/2003 Plasmid DNA isolation (maxi-prep)
10/16/2003 Transfection of J774 using FuGENE 6 reagent
10/18/2003 High Efficiency Transformation of Yeast Cells
10/18/2003 â-Galactosidase Staining of Eukaryotic Cells in vitro
10/19/2003 Crosslinking DNA-Binding Protein to DNA Using a Bromodeoxyuridine-Substituted Probe
10/19/2003 Baculovirus Transfection Using Calcium Chloride
10/21/2003 Labeling of DNA Probes using Primer Extension
10/21/2003 Phage Capture Assay: Quantifying Binding of Receptor to Phage Clones
10/21/2003 Transfection of Cells with Recombinant Phage DNA by Electroporation
10/22/2003 "Low Background" Phage Display Protocol for the Isolation of Protein-Binding Peptides
10/22/2003 Biotinylation of Antibodies in Phage Display
10/24/2003 Quickie Transformations
11/7/2003 Simplified Arabidopsis Transformation Protocol
11/10/2003 Agrobacterium plasmid DNA miniprep
11/10/2003 Introduction of plasmid into Agrobacterium
11/10/2003 Electroporation of A. tumåfaciens cells
11/10/2003 Making electrocompetent Agrobacterium
11/18/2003 High Efficency Yeast Plasmid Rescue
11/18/2003 Colony Plasmid Rescue
11/18/2003 High efficiency LiAc transformation
11/18/2003 Quick transformation
11/18/2003 PLATE Transformation
11/18/2003 Electroporation (Yeast)
11/18/2003 Gene Disruption via PCR (Yeast)
11/18/2003 Phenol/Freeze RNA Prep (Yeast)
11/18/2003 Glass Bead RNA Prep (Yeast)
11/18/2003 GST Fusion Protein Purification from Yeast
11/18/2003 2 Hybrid system screens
11/18/2003 Protocols for shuttle mutagenesis/epitope-tagging of a yeast gene with mTn-lacZ/LEU2
11/18/2003 EMS Mutagenesis of Yeast
11/18/2003 S1 nuclease assay using oligonucleotide probe
11/18/2003 Quick Change Mutagenesis
11/18/2003 Bacteriophage Plaque lifts
11/18/2003 Construction of homemade 'T-vectors'
11/18/2003 Generation of uni-directional nested deletions in plasmid DNA
11/18/2003 Library cDNA Synthesis
11/19/2003 Rubidium Chloride method for Transformation Competent E. coli
11/19/2003 Transformation "Ultra-Competent" E. coli (Inoue Method)
12/4/2003 YAC library - YAC DNA isolation procedures
12/4/2003 YAC library - Screening a YAC - library for positive clones with PCR
12/4/2003 YAC library - Pulse Field Gel Electrophoresis
12/4/2003 YAC library - Generation of YAC-ends by Inverse-PCR (IPCR)
12/4/2003 Cosmid library-Making glycerol stocks
12/4/2003 Cosmid library-Cosmid library screen using PCR
12/4/2003 Cosmid library-Cosmids pools screening
12/4/2003 Cosmid library-Large -scale prep of cosmids
12/4/2003 BIBAC library ISOLATION OF PLASMID DNA
12/4/2003 Subcloning inserts from traditional BAC or PAC vectors into the BIBAC
12/4/2003 [BIBAC] Electroporation protocols
12/4/2003 BIBAC LIBRARY Agrobacterium plasmid prep
12/4/2003 TOMATO TRANSFORMATION
12/4/2003 AGROBACTERIUM VIR GENE INDUCTION PROTOCOL
12/4/2003 Agrobacterium Transformation
12/4/2003 Construction of a genomic DNA library
5/15/2004 APOB EcoRI Polymorphism
5/15/2004 DRD2 HincII Polymorphism
5/15/2004 NGFR HindIII Polymorphism
5/15/2004 PAH PvuII B Polymorphism
5/15/2004 NGFR HincII Polymorphism
5/15/2004 PAH PvuII A Polymorphism
5/15/2004 SLC6A3 3' VNTR Polymorphism
5/15/2004 DRD2 TaqI "D" Polymorphism
5/15/2004 PAH AluI Polymorphism
11/2/2004 Calcium chloride Competent cells Preparation and transformation
11/17/2005 Easy 4 degree competent cells
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