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Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

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To comment on these protocols, please participate in the ongoing "Protocol Reviews" discussion in The Science Advisory Board's Forum.

 
DNA Purification
10/9/2003 Detection of Recombinant Proteins by ELISA
10/24/2003 DNA from plasma
11/7/2003 ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE
11/7/2003 NUCLEI ISOLATION
11/7/2003 CHLOROPLAST DNA ISOLATION
11/7/2003 RNA EXTRACTION METHOD FOR COTTON
11/10/2003 Genomic DNA Extraction - CTAB Protocol
11/10/2003 Genomic DNA Extraction - Quick Protocol
11/18/2003 Rapid Genomic Prep Yeast
11/18/2003 nup4a-313 Shuttling in Yeast
11/18/2003 De-phosphorylation of DNA
11/18/2003 Gene-Kleen protocol
11/18/2003 Hoechst Fluorometric quantitation of DNA
11/21/2003 PHAGE PURIFICATION WITH DE52
11/21/2003 Polysomal RNA isolation and purification
11/24/2003 Radioactive DNA Fragmentation Assay
11/24/2003 Radioactive Proliferation Assay
11/24/2003 DNA laddering assay for treated cells
11/24/2003 Fluorescence microscopy of DAPI stained cells
12/4/2003 10-min plasmid DNA miniprep method
12/4/2003 Maxi-plasmid DNA Prep
5/3/2004 C. elegans RNA prep
5/15/2004 DNA preparation by cryotom tissue dissection
5/15/2004 DNA detection CGH
5/15/2004 Hybridization FOR CGH
5/15/2004 D10S94 MspI Polymorphism
5/15/2004 HOXB4 MspI Polymorphism
5/15/2004 RET RsaI Polymorphism
5/15/2004 COMT NlaIII Polymorphism
5/15/2004 PLAT Alu Polymorphism
6/23/2004 Isolation Total genomic DNA from Peripheral Blood Leukocytes
6/24/2004 Safeguarding DNA against UV damage during isolation
6/27/2004 Elution of DNA fragments from agarose
6/1/2005 Small scale chromosomal DNA isolation from hair follicles
6/1/2005 Small scale chromosomal DNA isolation from brain tissue
2/12/2006 Plasmid Mini prep technique
10/27/2006 Quicky colony screen
3/27/2009 Even quicker colony screen for cloning
3/27/2009 Ultrafast Restriction Digest
3/27/2009 Freeze-Squeeze DNA Gel Extraction
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