Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

To comment on these protocols, please participate in the ongoing "Protocol Reviews" discussion in The Science Advisory Board's Forum.


RNA Methods
10/17/2003	RNA from tissues.
10/17/2003	Total cellular RNA
10/17/2003	EXTRACTION AND PURIFICATION OF TOTAL RNA USING TRIzol
10/19/2003	Native Gel Electrophoresis of RNA-Protein Complexes
10/19/2003	RNase Protection and Immunoprecipitation of Nuclear Extracts
10/20/2003	RNase Protection and Immunoprecipitation of Nuclear Extracts
10/20/2003	Deprotection of Synthetic RNA
10/20/2003	Reverse Transcriptase Assay Using DEAE-Cellulose
10/20/2003	RNA Primer Extension
10/21/2003	RNase Protection Assay
10/21/2003	Complete RNase T1 Digest
10/21/2003	RNase Protection and Immunoprecipitation of Nuclear Extracts
10/21/2003	In Vitro Transcription Assay (Run-off Assay) using Permeabilized Cells
11/7/2003	Methods for Plant RNA Isolation
11/10/2003	Isogen RNA Extraction Protocol
11/10/2003	Seed RNA Extraction Protocol
11/18/2003	Primer Extension analysis of total yeast RNA
11/18/2003	Single Stranded dut ung DNA Prep
11/18/2003	Yeast Chromatin Immunoprecipitation
11/18/2003	RNA synthesis from plasmid-borne sequences
11/19/2003	Ribonuclease Protection Assay
2/8/2004	RNAi in HeLa cells
6/29/2004	RNA denaturing gel - BTPE
10/6/2005	Isolation of total cytoplasmic RNA using LiCl-Urea extraction and PK digestion – Auffray and Rougeon modified method
7/1/2006	RNAi In Honeybee Brain
10/31/2006	In vitro transcription of radiolabelled RNAs
10/31/2006	Large-scale vitro transcription
10/31/2006	Preventing RNase contamination
3/14/2007	RNA isolation for Microarray

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