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Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

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Protein Purification
9/30/2003 Electrophoresis of DNA in Agarose Cells
10/1/2003 Acetylation (Or Succinylation) Of Amino Groups On Proteins
10/12/2003 Purification of His-tagged Proteins on Ni-NTA column
10/17/2003 Bradford Assay
10/17/2003 lowry protein estimation assay
10/17/2003 Seleno-methionine labelling of proteins in E. coli
10/17/2003 Immunoprecipitation using Protein A
10/17/2003 Affinity purification of antibodies
10/18/2003 Phycoerythrin conjugation protocol
10/21/2003 Preparation of Polysomes from E. coli
10/27/2003 Cell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity
10/27/2003 Cell Staining for Senescence-Associated â-Galactosidase (SA-â-Gal) Activity
11/10/2003 Extraction of Free Amino Acids Protocol
11/10/2003 S-adenosylmethionine (SAM) Extraction Protocol
11/17/2003 Insoluble Protein Purification
2/16/2004 Quick GST fusion protein purification
8/10/2006 Purification of prion proteins (SAFs)
10/23/2007 Quick and Easy Nuclear Extract

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