Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

To comment on these protocols, please participate in the ongoing "Protocol Reviews" discussion in The Science Advisory Board's Forum.


PCR and Related Methods
10/1/2003	Beta-Lactamase Induction (Crude Preparation)
10/19/2003	Gel Mobility Shift Assay Using Magnesium Chloride and EDTA
10/19/2003	Competitive Ligand Binding to Cortical Receptor using Crosslinking
11/10/2003	Inverse PCR Protocol
11/10/2003	Leaf GUS Assay
11/18/2003	Yeast Colony PCR
11/18/2003	Vectorette PCR of Yeast DNA
11/18/2003	UV Mutagenesis
11/18/2003	Disruption by Fusion PCR
11/18/2003	3'RACE PCR
11/18/2003	Ligation
11/21/2003	In vitro mutagenesis using Altered Sites
11/21/2003	Nested Deletions using exonuclease-III and mung bean nuclease
11/21/2003	Nested Deletions using exonuclease-III and mung bean nuclease
11/21/2003	DNA-se I FOOTPRINTING
11/21/2003	PRIMER LABELLING FOR PRIMER EXTENSION ASSAY and PRIMER EXTENSION
11/21/2003	OLIGO PURIFICATION on acrylamide gels
12/4/2003	Tail PCR
2/1/2004	PCR of GC rich templates
6/4/2004	RAPD ( Randomly Amplified Polymorphic DNA)
6/4/2004	Differential Display PCR
6/7/2004	Cloning of DNA fragments
6/7/2004	Mini prep and restriction digest conformation
6/7/2004	DNA precipitation
2/9/2006	Site directed mutagenesis
8/10/2006	Vectorette PCR for cloning unknown 5' or 3' flanking sequences
8/10/2006	Site-directed mutagenesis
10/28/2006	Inter-Retrotransposon Amplification Polymorphism (IRAP)
11/5/2006	Retrotransposon-Microsatellite Amplified Polymorphism (REMAP)
1/22/2008	Simplified RAPD (Random Amplified Polymorphic DNA) protocol
3/27/2009	Site-directed mutagenesis by fusion PCR

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