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Protocols

Welcome to The Science Advisory Board's extensive database of research protocols for the biomedical sciences. Protocols are organized by techniques and are fully searchable by key word(s). Protocols are divided into a brief description of the methods, a step-by-step overview of the procedure, a collection of recipes, the supplies needed, and any helpful tips. Members submitting protocols will be rewarded through the ViewPoints system.

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To comment on these protocols, please participate in the ongoing "Protocol Reviews" discussion in The Science Advisory Board's Forum.

 
Cell and Tissue Culture
9/22/2003 Phenol extraction of DNA samples  
9/30/2003 Northern Blots
10/1/2003 Alkaline Lysis Plasmid Miniprep
10/16/2003 Chromosome preparation and Fluorescence in-situ hybridization (FISH)
10/16/2003 Transformation of Listeria monocytogenes
10/17/2003 H-Thymidine Uptake by Cultured Cells
10/18/2003 Intracellular Cytokine Staining
10/18/2003 Matrigel invasion assays
10/18/2003 Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
10/19/2003 Measurement of Incorporation of 35S-Cysteine into Cellular Protein
10/19/2003 Overexpression of Recombinant Proteins in COS-7 Cells
10/20/2003 Polymerase III In Vitro Transcription
10/20/2003 In Vitro Transcription of RNA
10/20/2003 In Vitro Transcription with SP6 Polymerase
10/21/2003 Labeling Oligonucleotides
10/21/2003 Chemotaxis Assay Using Microchemotaxis Chambers (Modified Boyden Chamber)
10/21/2003 Human Peripheral Blood Mononuclear Cell Preparation
10/21/2003 TransWell Chemotaxis
10/27/2003 G1/S Phase Block for Cell Synchronization
10/27/2003 G2 Phase Block with Hoechst 33342 for Cell Synchronization
10/27/2003 Metaphase Block for Cell Synchronization
10/27/2003 Preparing Chromosome Spreads
11/7/2003 MITOCHONDRIAL DNA ISOLATION
11/7/2003 Green lab protocol for vacuum infiltration transformation of Arabidopsis
11/7/2003 Cell Suspension Culture of Arabidopsis
11/10/2003 Plant Crosse Protocol
11/10/2003 Protocol for determination of seed moisture content
11/13/2003 In vitro growth of seedlings
11/13/2003 Preparation of Solid Tissue for Aspergillus Galactomannan
11/18/2003 Yeast Prep for FACS
11/18/2003 Pheromone Halo Assay
11/18/2003 PEG Preparation of Plasmid DNA
11/19/2003 Rapid PCR Screening of pRSET bacterial colonies
11/21/2003 Isolate physiologically active thylakoid membranes
11/21/2003 Protein Expression Screening Using Plant Tissue Prints On Nitrocellulose
11/21/2003 Isolation of Chloroplasts from Spinach Leaves
11/21/2003 Lyophilizing Cells
11/24/2003 Giemsa for blood cells and parasites
11/24/2003 Leishman's stain for blood cells and parasites
11/24/2003 Acridine Orange Stain
11/24/2003 Gentle lysis of cells in sucrose-containing Mito-Buffer
11/24/2003 Culture of Human Prostatic Carcinoma Cell Lines
11/24/2003 Electrophoretic Mobility Shift Assay (EMSA) for the detection of nuclear NF-kB
12/4/2003 ARABIDOPSIS ROOT TRANSFORMATION ASSAYS
12/4/2003 Agrobacterium-mediated Tobacco Transformation
12/4/2003 Rice Bombardment
12/4/2003 PP2C assay
12/4/2003 Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts : Protoplast Isolation
12/4/2003 Transient Expression Assay Using Arabidopsis Mesophyll Protoplasts : PEG Transfection
12/4/2003 Transient Expression Assay Using Maize Mesophyll Protoplasts
12/4/2003 Histochemical GUS assay
12/4/2003 Cysteine proteinase activity assay
12/4/2003 Western blot
12/19/2003 Spermatozoa Chemotaxis assay using Zigmond Chamber.
1/17/2004 Determination of IC50
1/29/2004 G1/S cell synchnronization using double thymidine block
3/16/2004 Wright's staining
3/16/2004 Cell counts
3/16/2004 Sézary Cell Preparation
3/16/2004 Freezing of Cells with Serum
5/3/2004 Culture of Dissociated Sympathetic Neurons
5/3/2004 Culture of Dissociated Cortical Neurons
5/3/2004 Conditioned Medium for Retinal Explant Culture
5/3/2004 Organotypic Culture
5/3/2004 Preparation of a dispersed neuron culture of rat suprachiasmatic nucleus (SCN)
5/3/2004 Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
5/3/2004 Dissociated Retina Immunohistochemistry
5/3/2004 Antigen Retrieval
5/3/2004 PEROXIDASE-ANTI-PEROXIDASE TECHNIQUE FOR THE IDENTIFICATION OF ANTIGENS IN PARAFFIN WAX SECTIONS
5/3/2004 Cytokine-Induced Proliferation of Indicator Cell Lines
5/3/2004 TNF-alpha-Induced Killing of L929 Cell Line
5/3/2004 IFN-gamma Protection from Viral Infection of Cell Lines
5/3/2004 Cytokine ELISPOT Protocol
5/3/2004 Antibody Neutralization of Cytokine-induced Proliferation of Indicator Cell Lines
5/3/2004 Antibody Neutralization of TNFa-Induced Killing of L929 Cell Line
5/3/2004 Antibody Neutralization of IFNg-Protection from Viral Infection of L929 and A549 Cell Lines
5/5/2004 Pediatric Virus-specific Bulk Stimulation Cytotoxic T Lymphocyte (CTL) Protocol
5/5/2004 HUMAN SOLUBLE INTERLEUKIN-2 RECEPTOR "
5/5/2004 DETECTION OF ISOTYPE SPECIFIC IgG ANTIBODIES
5/5/2004 ELISA, Antibody capture
5/5/2004 Sandwich ELISA for SCCA1 and SCCA2
5/5/2004 NEOPTERIN ENZYME IMMUNOASSAY (EIA) TEST PROCEDURE
5/5/2004 Setting up 2 or 3 Colour FACS analysis
5/5/2004 Yeast FACS
5/5/2004 Immunofluorescence Yeast
5/10/2004 Iindirect immunoflourescence for yeast:
5/10/2004 IIF Protocol (modifications for maintaining GFP fluorescence
5/10/2004 PDS1::HA IMMUNOFLUORESCENCE
5/10/2004 Antibody Conjugation
5/10/2004 Staining for Surface Receptors
5/10/2004 Staining for total receptors
5/10/2004 Yeast IF with Methanol/Acetone Dehydration
5/10/2004 Yeast IF without Dehydration
5/10/2004 RESPONDER CELL FREQUENCY ASSAY (RCF): LPA – Limiting Dilution
5/10/2004 WHOLE BLOOD LYMPHOCYTE PROLIFERATION ASSAY
5/10/2004 Anti-TLR4 Neutralization of LPS-induced Cytokine Production by Human PBMC
5/10/2004 Anti-TLR4/MD2 Neutralization of Lipid A-induced Cytokine Production by Mouse Peritoneal Cells
5/10/2004 Protocol for RBC Lysis of Mouse Spleen
5/15/2004 Metaphase chromosome preparation
5/15/2004 ADH2 Intron 3 RsaI Polymorphism
5/15/2004 HOXB3 TaqI Polymorphism
5/15/2004 PAH MspI Polymorphism
5/15/2004 RET TaqI Polymorphism
5/15/2004 PAH BglII Polymorphism
5/15/2004 COMT BglI Polymorphism
5/15/2004 APOB XbaI Polymorphism
5/15/2004 PAH XmnI Polymorphism
6/7/2004 Cloning cells by limiting dilution
6/29/2004 How to freeze mamalian cells
9/10/2004 Mitochondria staining(Mito Tracker Red CM-H2Xros)
4/30/2005 Bulk synthetic RNA preparation
5/4/2005 Cell Clonogenic Survival Assay
5/18/2005 Protein extraction for SDS-PAGE from human monocytes
6/1/2005 Primary neuron viability
6/12/2005 Dorsal Root Ganglion Neuron Culture from Adult Rats
6/14/2005 Differentiation of 3T3-L1 fibroblasts to adipocytes
3/24/2006 Bioassay BMP-2
6/1/2006 Sodium Orthovanadate Activation
6/1/2006 Immunoprecipitation protocol for Mammalian cells
6/21/2006 Flow cytometry for Intracellular Antigens
7/24/2006 Acridine orange /propidium iodide double staining for apoptotic and necrotic cell determination
8/20/2006 Obtention and culture of mouse peritoneal macrophages
10/22/2006 Erythrosin B for Cell Viability Determinations
10/30/2006 Cell Counting and Viability Using Fluorescein Diacetate (FDA)
10/30/2006 Annexin V Apoptosis Assay by Flow Cytometry
10/30/2006 JC-1 Apoptosis Assay by Flow Cytometry
10/30/2006 Detection of Phosphorylated Proteins by Flow Cytometry
10/31/2006 Measurement of Alkaline Phosphatase activity in cell cultures
10/31/2006 COLLAGEN ASSAY USING THE PICRO-SIRIUS RED METHOD
10/31/2006 Measuring DNA in cell cultures using Hoescht 33258 dye
11/27/2006 293 FreeStyle Transfection and Pierce Purifcation of Antibody (IgG)
11/29/2006 Growth, Maintenance and Transfection of Suspension Adapted 293-EBNA cells
11/29/2006 Qiagen HiSpeed Plasmid Maxi Kit
12/1/2006 Growth and Maintenance of FreeStyle 293-F cells
12/1/2006 293fectin transfection FreeStyle 293-F cells (antibody)
12/1/2006 Western Blot sample prep from cell culture
12/18/2006 PCR Detection of Mycoplasmae contaminants
1/23/2007 Tube formation assay
1/26/2007 Endothelial wound healing (cell migration) assay
2/10/2007 Noble Agar Assay
2/22/2007 Enrichment of PBMCs with monocytes
3/23/2007 ROS determination
6/7/2007 Procedure for fixing and staining adherent cells for immunofluorescence
11/11/2007 Motility Assay
11/11/2007 Hanging drop aggregation assay
7/6/2010 PEI transfection of HEK cells
7/29/2010 Production of Mouse T Cell Hybridomas
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