Understanding Fundamental Cellular Processes
Stanislav Naryzhny, Ph.D.
A Member Since October 1997
Trained as a radiobiologist, Stanislav Naryzhny, Ph.D. went to work at the St. Petersburg Nuclear Physics Institute (PNPI) after obtaining both his Bachelor and Master degrees in biophysics from Tbilisi State University in Georgia. Although his doctoral thesis was awarded from the Biophysics Institute in Pushchino, Russia, all of his experiments were conducted at the PNPI. Dr. Naryzhny studied the molecular mechanisms involved in DNA repair, focusing specifically on its structural aspects. He investigated how the chromatin structure itself served an important role in orchestrating the enzymes involved in fixing damaged DNA.
Because it was difficult to conduct science in Russia, mainly due to lack of funding, Dr. Naryzhny moved to Canada. He currently holds the title of Associate Scientist, which he earned after completing a postdoctoral fellowship at the Northeastern Ontario Regional Cancer Centre in Sudbury. His research is dedicated towards developing a better understanding of the biochemical mechanisms of cell-cycle regulation in eukaryotes.
He studies the enzymology of the cell cycle by first taking a proteomics approach. He narrows down the proteins of interest by performing two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) before analyzing specific proteins via cell culture experiments using hamster and some human cell lines. Dr. Naryzhny is currently investigating proliferating cell nuclear antigen (PCNA). PCNA is involved in the regulation of various biological functions, including DNA replication, DNA repair, cell cycle and chromatin remodeling. It looks like PCNA is a key protein that coordinates these different processes by a striking ability to interact with multiple partners.
Dr. Naryzhny has extensive hands-on experience in protein purification using different electrophoretic and chromatographic techniques. In fact, he holds two patents on electrophoretic separation techniques. He is also highly skilled in protein detection after 2-D PAGE according to their activity, by immunoblotting and other approaches. While in Russia he developed an expertise in the application of proteomics approaches for the classification, purification and analysis of industrial enzymes. As a child, his interest in science was piqued by his desire to “discover something new every day.”
An avid photographer, Dr. Naryzhny is enjoying exploring new places and taking pictures of its natural beauty. He recently returned from a two-week vacation to Nova Scotia and was particularly impressed with Cape Breton Island. He is a chess player who also enjoys physical activity playing soccer or tennis.
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